Target molecule attachment to surfaces

Details Target molecule attachment to surfaces Target molecule attachment to surfaces

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07B 61/00 (2006.01) C07H 21/00 (2006.01)

Patent

CA 2360000

Method and reagent composition for covalent attachment of target molecules, such as nucleic acids, onto the surface of a substrate. The reagent composition includes groups capable of covalently binding to the target molecule. Optionally, the composition can contain photoreactive groups for use in attaching the reagent composition to the surface. The reagent composition can be used to provide activated slides for use in preparing microarrays of nucleic acids.

L'invention concerne un procédé et une composition de réactif, destinés à la fixation par liaison covalente, de molécules cibles, telles que des acides nucléiques, sur la surface d'un substrat. La composition de réactif comprend des groupes capables de se fixer, par liaison covalente, à la molécule cible. La composition peut éventuellement comprendre des groupes photoréactifs, utilisés pour la fixation de la composition de réactif à la surface. On peut utiliser la composition de réactif pour produire des lames activées, utilisées pour la préparation de jeux ordonnés de microéchantillons d'acides nucléiques.

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