Target nucleic acid amplification/detection systems

Details Target nucleic acid amplification/detection systems Target nucleic acid amplification/detection systems

C - Chemistry, Metallurgy

12

N

195/1.12, 150/8.

C12N 15/00 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2004574

ABSTRACT OF THE DISCLOSURE TARGET NUCLEIC ACID AMPLIFICATION/DETECTION SYSTEMS This invention relates to the use of functional reporter molecules in the detection and measurement of RNA sequences in a sample, as a determination, for example, of pathogenic disease existence or potential. The invention is predicated on the utilization of nucleotide sequences, one having a probe sequence linked to the complement of a sequence capable of initiating replication. The other is capable of hybridizing to a strand separated from the extension product of the first nucleotide sequence after hybridization to a specific target sequence. The extension product of the second hybridized nucleotide sequence serves as a template source for replication. The replication products are detected as a means for detection of target sequence.

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