Target plate for mass spectometers and use thereof

Details Target plate for mass spectometers and use thereof Target plate for mass spectometers and use thereof

H - Electricity

01

J

H01J 49/40 (2006.01) B01L 3/00 (2006.01) B01L 3/02 (2006.01) G01N 27/447 (2006.01) G01N 35/02 (2006.01) H01J 49/04 (2006.01) G01N 1/34 (2006.01) G01N 1/40 (2006.01) G01N 35/00 (2006.01) G01N 35/10 (2006.01)

Patent

CA 2469841

A plate suitable for use with mass spectrometers comprising a number of target spots arranged in a surface portion of said plate making it possible to deposit small amounts of fluid at said target spots without the fluid escaping or getting mixed with fluid deposited at another target surface of the same plate. The target surfaces being arranged in a surface portion of said plate so that a base material of said plate constitutes the walls of receptacles, characterised in that the shape, size, temperature and possible agents of said receptacle facilitate evaporation of a solution in which sample molecules are suspended. Embodiments include different types of matrix and enzymes arranged at said spots, and methods for enhancing MALDI analysis efficiency.

L'invention concerne une plaque que l'on peut utiliser avec des spectromètres de masse, qui comprend un certain nombre de points cibles disposés sur une partie de sa surface, ce qui permet de déposer de petites quantités de liquide sur lesdits points cibles sans que le liquide s'échappe ou se mélange avec le liquide déposé sur une autre surface cible de la même plaque. Les surfaces cibles sont disposées sur une partie de la surface de ladite plaque de façon qu'un matériau de base de cette plaque forme les parois de réceptacles, la forme, la taille, la température et d'éventuels agent desdits réceptacles facilitant l'évaporation d'une solution dans laquelle des molécules échantillons sont en suspension. Des modes de selon l'invention portent notamment sur différents types de matrices et d'enzymes disposés sur les points en question, ainsi que sur des procédés d'amélioration de l'efficacité de l'analyse MALDI.

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