Targeted chromosomal genomic alterations in plants using...

Details Targeted chromosomal genomic alterations in plants using... Targeted chromosomal genomic alterations in plants using...

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) A01H 5/00 (2006.01) C07H 21/00 (2006.01) C12N 5/10 (2006.01) C12N 15/11 (2006.01) C12N 15/82 (2006.01) C12Q 1/68 (2006.01) A61K 38/00 (2006.01) A61K 48/00 (2006.01)

Patent

CA 2410523

Presented are methods and compositions for targeted chromosomal genomic alterations with modified single-stranded oligonucleotides. The oligonucleotides of the invention have modified nuclease-resisant termini comprising LNA, phosphorothioate linkages or 2'-O-Me base analogues or combinations of such modifications.

L'invention concerne des procédés et des compositions servant à provoquer des modifications génomiques chromosomiques ciblées au moyen d'oligonucléotides monocaténaires modifiés. Ces oligonucléotides possèdent des terminaisons modifiées résistantes à la nucléase comprenant LNA, des liaisons de phosphorothioate ou des analogues de bases 2'-O-Me ou des combinaisons de ces modifications.

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