Targeted chromosomal mutagenesis using zinc finger nucleases

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/90 (2006.01) C12N 9/22 (2006.01) C12N 15/01 (2006.01) C12N 15/09 (2006.01) C12N 15/79 (2006.01) C12N 15/82 (2006.01) C12N 15/87 (2006.01)

Patent

CA 2474486

The present invention provides for a method or methods of targeted genetic recombination or mutagenesis in a host cell or organism, and compositions useful for carrying out the method. The targeting method of the present invention exploits endogenous cellular mechanisms for homologous recombination and repair of double stranded breaks in genetic material. The present provides numerous improvements over previous mutagenesis methods, such advantages including that the method is generally applicable to a wide variety of organisms, the method is targeted so that the disadvantages associated with random insertion of DNA in to host genetic material are eliminated, and certain embodiments require relatively little manipulation of the host genetic material for success. Additionally, it provides a method that produces organisms with specific gene modifications in a short period of time.

L'invention a trait à un procédé de mutagenèse ou de recombinaison génétique ciblée dans une cellule hôte ou organisme, et à des compositions utilisées dans ce procédé. Ce procédé de ciblage exploite des mécanismes cellulaires endogènes de façon à obtenir une réparation et une recombinaison homologue des ruptures à double brin de matière génétique. Ce procédé se caractérise notamment en ce qu'il peut être en général utilisé sur un grand éventail d'organismes, en ce qu'il est ciblé de sorte qu'il ne présente pas les désavantages associés à l'insertion aléatoire d'ADN dans une matière génétique hôte, et en ce que, dans certains modes de réalisation, la manipulation de la matière génétique hôte est sensiblement petite. De plus, l'invention concerne un procédé qui produit des organismes ayant des modifications génétiques spécifiques à court terme.

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