Targeted cytolysis of hiv-infected cells by chimeric cd4...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/62 (2006.01) A61K 35/12 (2006.01) A61K 35/14 (2006.01) A61K 35/28 (2006.01) A61K 35/54 (2006.01) A61P 31/18 (2006.01) C07K 14/73 (2006.01) C07K 19/00 (2006.01) C12N 5/10 (2006.01) C12N 15/12 (2006.01)

Patent

CA 2182890

Disclosed is a method of directing a cellular immune response against an HlV-infected cell in a mammal involving a administering to the mammal an effective amount of therapeutic cells which express a membrane-bound, proteinaceous chimeric receptor comprising (a) an extracellular portion which includes a fragment of CD4 which is capable of specifically recognizing and binding the HIV-infected cell but which does not mediate HIV infection and (b) an intracellular portion which is capable of signalling the therapeutic cell to destroy the receptor-bound HIV-infected cell. Also disclosed are cells which express the chimeric receptors and DNA and vectors encoding the chimeric receptors.

Procédé permettant d'orienter une réponse immunitaire cellulaire contre des cellules infectées par le VIH chez un mammifère, qui consiste à administrer à ce mammifère une dose efficace de cellules thérapeutiques exprimant un récepteur chimère protéique lié à la membrane. Ledit récepteur comprend (a) une partie extracellulaire qui contient un fragment de CD4 capable de reconnaître spécifiquement la cellule infectée par le VIH et de s'y lier, mais qui n'induit pas l'infection à VIH, et (b) une partie intracellulaire capable de signaler à la cellule thérapeutique de détruire la cellule infectée par le VIH et liée au récepteur. Des cellules qui expriment les récepteurs chimères et de l'ADN et des vecteurs codant lesdits récepteurs chimères sont également décrits.

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