Targeted split biomolecular conjugates for the treatment of...

A - Human Necessities – 61 – K

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A61K 47/48 (2006.01) A61K 49/00 (2006.01) A61P 1/16 (2006.01) A61P 9/00 (2006.01) A61P 25/00 (2006.01) A61P 29/00 (2006.01) A61P 31/00 (2006.01) A61P 35/00 (2006.01)

Patent

CA 2667621

The present invention is directed to compositions and methods for the production of split-biomolecular conjugates for the directed targeting of nucleic acids and polypeptides. More preferably, the compositions and methods allow for the use of the split biomolecular conjugates for the treatment of diseases, malignancies, disorders and screening. In some embodiments, the split biomolecular conjugates comprise split effector protein fragments conjugated to a probe, and interaction of both probes with a target nucleic acid or target polypeptide, such as a pathogenic nucleic acid sequence or pathogenic protein, brings a the split-effector fragments together to facilitate the reassembly of the effector molecule. Depending on the effector molecule, the protein complementation results in a cellular effect, in particular for the treatment of diseases, malignancies and disorders.

Cette invention se rapporte à des compositions et à des procédés de production de conjugués biomoléculaires fractionnés utilisés pour le ciblage dirigé d'acides nucléiques et de polypeptides. De préférence, les compositions et les procédés permettent d'utiliser les conjugués biomoléculaires fractionnés dans le traitement de maladies, de tumeurs malignes, d'affections et dans le dépistage. Dans certains modes de réalisation, les conjugués biomoléculaires fractionnés comprennent des fragments protéiniques effecteurs fractionnés conjugués à une sonde, et à l'interaction des sondes avec un acide nucléique cible ou un polypeptide cible, par exemple une séquence d'acides nucléiques pathogène ou une protéine pathogène, rassemble les fragments effecteurs fractionnés et faciliter le regroupement de la molécule effectrice. Selon la molécule effectrice, l'association protéinique aboutit à un effet cellulaire, en particulier dans le traitement de maladies, de tumeurs malignes et d'affections.

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