Targeted tumor therapy by use of recombinant adenovirus...

A - Human Necessities – 61 – K

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Details

A61K 48/00 (2006.01) C07K 14/075 (2006.01) C12N 5/10 (2006.01) C12N 15/86 (2006.01) C12N 15/861 (2006.01)

Patent

CA 2501010

The presently claimed subject matter provides conditionally replication competent adenoviral vectors that confer selective cytotoxicity on cells expressing HIF-1 by infecting cells that allow HIF-1 inducible promoters present within the vectors to function. Also provided are compositions and host cells based upon the vectors, as well as methods of propagating and using the vectors. The presently claimed subject matter further provides a method of inhibiting tumor growth by co-infecting cells in a tumor with a conditionally replication competent adenovirus vector in conjunction with a replication deficient adenovirus vector.

L'invention concerne des vecteurs adénoviraux responsables de la réplication sous certaines conditions, qui confèrent une cytotoxicité sélective aux cellules exprimant HIF-1 par l'infection des cellules qui permettent de fonctionner aux promoteurs inductibles par HIF-1 présents dans les vecteurs. L'invention concerne aussi des compositions et des cellules hôtes basés sur les vecteurs ainsi que des procédés de propagation et d'utilisation des vecteurs. L'invention concerne également un procédé pour inhiber la croissance tumorale au moyen de la co-infection des cellules dans une tumeur en utilisant un vecteur d'adénovirus responsable de la réplication sous certaines conditions, conjointement avec un vecteur d'adénovirus incapable de réplication.

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