Targeting and tracing of antigens in living cells

Details Targeting and tracing of antigens in living cells Targeting and tracing of antigens in living cells

C - Chemistry, Metallurgy

07

K

C07K 19/00 (2006.01) C07K 16/46 (2006.01)

Patent

CA 2628388

The present invention relates to a method of detecting the presence, amount or subcellular location of an antigenic structure of interest in a cell, comprising the steps of : (a) (i) expressing a fusion protein directed to the antigenic structure of interest in said cell or (ii) introducing a fusion protein directed to the antigenic structure of interest and coupled to a (poly) peptide capable of transducing into said cell; wherein said fusion protein comprises a first (poly) peptide sequence comprising the variable region of a heavy chain antibody of Camelidae and a second (poly) peptide sequence derivable from a fluorescent or chromophoric protein.

La présente invention concerne un procédé permettant de détecter la présence, la quantité ou l'emplacement subcellulaire d'une structure étudiée dans une cellule. Ce procédé consiste à (i) exprimer une protéine de fusion dirigée contre la structure antigène étudiée dans la cellule ou (ii) à introduire une protéine de fusion dirigée contre la structure antigène étudiée et couplée à un (poly)peptide capable de transduction donnant ladite cellule. En l'occurrence, cette protéine de fusion comporte une première séquence (poly)peptide comprenant la région variable d'un anticorps à chaîne lourde de Camelidae et une deuxième séquence (poly)peptide pouvant dériver d'une protéine fluorescente ou chromophore.

Affiliated with

Also associated with

Rating

Targeting and tracing of antigens in living cells does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Targeting and tracing of antigens in living cells, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Targeting and tracing of antigens in living cells will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1451375