Tbg assay

Details Tbg assay Tbg assay

C - Chemistry, Metallurgy

07

K

150/15, 195/35

C07K 17/00 (2006.01) C12N 11/00 (2006.01) C12Q 1/00 (2006.01) C12Q 1/25 (2006.01) G01N 33/78 (2006.01)

Patent

CA 1096793

ABSTRACT OF THE DISCLOSURE Method and compositions are provided for deter- mining the thyroxine binding capacity of serum the value finding u e in determinations of free thyroxine index. A competitive assay is perfomed where antibody for T-3 (anti- triiodothyronine) and serum thyroxine binding sites compete for T-3 (triiodothyronine) in the assay mediums the T-3 is present as free T-3 added in a predetermined amount and T-3 conjugated to an enzyme. By combining the materials in a buffered aqueous medium with enzyme substrates, and de- termining the rate of reaction by the changing concentration of a light absorptive substance over a predetermined period, the thyroxine binding capacity of the serum can be deter- mined.

299336

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