Techniques for identifying, confirming, mapping and...

C - Chemistry – Metallurgy – 40 – B

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C40B 30/00 (2006.01) C12N 15/09 (2006.01) C12Q 1/68 (2006.01) C40B 30/04 (2006.01)

Patent

CA 2302193

Systems and methods for identifying, confirming, mapping, and categorizing sample polymers, such as nucleic acid sequences, are provided. An estimation of the fraction of first and second polymers in a sample of polymers can be calculated by inputting a first hybridization value indicative of hybridization affinity of the sample of polymers to polymers probes that are complementary to the first polymer and inputting a second hybridization value indicative of hybridization affinity of the sample of polymers to polymers probes that are complementary to the second polymer. The estimation of the fraction of the first and second polymers in the sample of polymers can then be calculated by dividing the first hybridization value by a sum of the first and second hybridization values. Estimations of the fractions of alleles in a sample can be clustered to form a fraction pattern usable for identifying, confirming, mapping, and genotyping sample nucleic acids.

L'invention concerne des systèmes et méthodes qui permettent d'identifier, de confirmer, d'agencer et de cartographier des polymères types tels que des séquences d'acides nucléiques. Il est possible de calculer une estimation de la fraction des premier et second polymères dans un échantillon de polymères en entrant une première valeur d'hybridation indicative de l'affinité à l'hybridation de cet échantillon avec des sondes polymères complémentaires du premier polymère, puis une seconde valeur d'hybridation indicative de l'affinité à l'hybridation de l'échantillon de polymères avec des sondes polymères complémentaires du second polymère. On pourra alors calculer la fraction des premier et second polymères dans l'échantillon de polymères en divisant la première valeur d'hybridation par la somme des première et seconde valeurs d'hybridation. Les estimations des fractions d' allèles peuvent être réunies et servir de schéma de fraction pour identifier, confirmer, cartographier et faire le génotypage d'acides nucléiques types.

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