Temperature dependent protein purification

Details Temperature dependent protein purification Temperature dependent protein purification

C - Chemistry, Metallurgy

07

K

C07K 1/22 (2006.01) C07K 14/315 (2006.01) C07K 16/06 (2006.01) C07K 16/12 (2006.01)

Patent

CA 2341498

The invention concerns the use of a coiled coil protein which is capable of forming a homodimer which dissociates into monomers on change of temperature, in a method of isolating a ligand or desired protein from a sample. A method of isolating a ligand which binds to the coiled coil protein is described as well as methods of isolating a desired protein which is tagged with a coiled coil protein or a ligand which binds to a coiled coil protein, or is tagged with a coiled coil protein monomer.

Cette invention a trait à l'emploi d'une protéine bispiralée capable de former un homodimère se dissociant en monomères sous l'effet d'une variation de la température, cette protéine étant utilisée dans le cadre d'un procédé d'isolation d'un ligand ou d'une protéine souhaitée dans un échantillon. L'invention porte également sur un procédé d'isolation d'un ligand se fixant à la protéine bispiralée ainsi que sur des procédés d'isolation d'une protéine souhaitée qui est marquée par une protéine bispiralée ou par un ligand se fixant à la protéine bispiralée ou encore par un monomère de protéine bispiralée.

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