Temperature sensitive multicopy plasmids

Details Temperature sensitive multicopy plasmids Temperature sensitive multicopy plasmids

C - Chemistry, Metallurgy

12

N

195/1.27, 195/1.

C12N 1/20 (2006.01) C07K 14/565 (2006.01) C12N 15/00 (2006.01) C12N 15/69 (2006.01) C12N 15/70 (2006.01)

Patent

CA 1198069

TEMPERATURE SENSITIVE MULTICOPY PLASMIDS ABSTRACT A plasmid is described of ColEl type parentage which has a temperature sensitive replication system such that the plasmid copy number in a given host cell may be controlled by a temperature shift. The plasmid permits the use of temperature as a switch for controlling gene amplification and hence production of corresponding protein in a cell.

429740

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