C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
C12P 19/34 (2006.01) C12N 15/10 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2222510
The present invention relates to methods of enzymatically synthesizing chirally pure oligonucleotides. In particular, it relates to methods of enzymatically synthesizing oligonucleotides using optionally digestible templates which are capable of directing the synthesis of an oligonucleotide product which can optionally be cleaved into two or more individual oligonucleotides, optionally using 3'-ribonucleotide primers. In this manner, more than one oligonucleotide having the same or different length and/or sequence can be simultaneously synthesized. The oligonucleotides optionally have at least one phosphorothioate linkage, and contain certain restriction endonuclease recognition sites herein identified as cleavable within phosphorothioate oligonucleotides, thus facilitating the separation and purification of the oligonucleotides after synthesis.
L'invention porte sur des procédés de synthèse enzymatique d'oligonucléotides chiralement purs en particulier en utilisant des matrices facultativement digestibles capables d'orienter la synthèse des oligonucléotides produits qui peuvent facultativement être scindés en deux ou plusieurs oligonucléotides individuels en utilisant des amorces de 3'-ribonucléotides. De cette manière, on peut synthétiser simultanément plus d'un oligonucléotide présentant une longueur et/ou une séquence identique ou différente. Les oligonucléotides peuvent présenter facultativement au moins une liaison phosphorothioate et contenir certains sites de reconnaissance d'endonucléases de restriction identifiés ici comme scindables à l'intérieur d'oligonucléotides phosphorothioatés, ce qui facilite la séparation des oligonucléotides après leur synthèse.
Dattagupta Nanibhushan
Kacian Daniel Louis
Lackey David Bruce
Gen-Probe Incorporated
Smart & Biggar
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