Template-directed photoligation

Details Template-directed photoligation Template-directed photoligation

C - Chemistry, Metallurgy

12

Q

195/1.12, 150/8.

C12Q 1/68 (2006.01) C07D 311/14 (2006.01) C07D 311/16 (2006.01) C07D 311/18 (2006.01) C07D 493/04 (2006.01) G01N 33/532 (2006.01)

Patent

CA 1339304

Methods, apparatus and compositions are presented for ligating ligands together which bind to a common target. One embodiment includes polynucleotide probes having photoreactive functional groups. The probes are capable of assuming substantially contiguous reactive positions on a target polynucleotide placing the photoreactive groups in juxtaposition. Activation of the photoreactive functional groups with radiant energy form a probe reaction product in which the probes are bound to each other.

586425

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