Test article and method for performing blood coagulation assays

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/56 (2006.01) C12M 1/40 (2006.01) G01N 33/52 (2006.01) G01N 33/86 (2006.01)

Patent

CA 2133983

A test article for determining coagulation capability in a blood sample comprises a porous membrane having a coagulation initiator and substrate impregnated therein. The pore dimensions and composition of the membrane are selected so that only blood plasma can pass into the interior of the membrane, where coagulation is initiated. The substrate is activated by a component of the coagulation pathway, typically thrombin, and produces a detectable signal upon activation. By utilising membrane matrix materials which are substantially free from interference with the coagulation pathway, accurate results can be achieved.

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