Test composition and method for the determination of anilides

Details Test composition and method for the determination of anilides Test composition and method for the determination of anilides

C - Chemistry, Metallurgy

12

Q

150/11, 150/14,

C12Q 1/34 (2006.01) C12M 1/40 (2006.01) C12Q 1/28 (2006.01)

Patent

CA 1321945

ABSTRACT OF THE DISCLOSURE A method and unitized test composition is described for the estimation of an anilide in which the enzymatic hydrolysis of the anilide and colorimetric quantitation of aniline or aniline derivative can be done simultaneously. The hydrolysis of the anilide is catalyzed by a known enzyme, arylacylamidase, E. C. 3.5.1.13. Stabilization of the enzyme is provided by the addition of controlled amounts of a compound containing alcoholic and/or aromatic groups such as ortho-cresol, isopropanol or benzoate. Basically, the unitized test composition comprises (i) arylacylamidase, (ii) a controlled amount of an organic compound containing alcoholic and/or aromatic groups which acts as both a stabilizer for the arylacylamidase and forms a colored product with aniline, and (iii) a novel oxidant/catalytic agent for accelerating color development. In addition, a method for the stabilization of arylacylamidase enzyme is described.

581268

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