G - Physics – 01 – N
Patent
G - Physics
01
N
167/43, 150/9
G01N 33/53 (2006.01) B01L 3/00 (2006.01) G01N 33/543 (2006.01) G01N 33/74 (2006.01) G01N 33/76 (2006.01) G01N 37/00 (2006.01)
Patent
CA 1340920
The present invention provides a method and a test cell for detecting a ligand in a liquid sample. According to the method of the present invention a solution is transported along a flow path in the test cell. The solution is brought into contact with a test site visible through a window in a wall of the test cell. The solution includes a liquid sample suspected to contain a ligand and a conjugate. The test site has immobilized thereon a first protein having a binding site specific to a first epitope on the ligand. The conjugate comprises coloured particles coupled to a second protein selected from the group consisting of proteins having a binding site specific to a second epitope on the ligand and proteins which bind with the first protein in competition with the ligand. The solution is then transported to progressively produce at the test site a complex comprising the ligand for a time sufficient to visually determine through the window whether a colour is developed at the test site.
604025
Charlton David E.
Miller Neal W.
Carter-Wallace Inc.
Dennison Associates
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