C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/10 (2006.01) A01H 5/00 (2006.01) A61K 39/385 (2006.01) C07K 14/415 (2006.01) C07K 16/16 (2006.01) G01N 33/53 (2006.01) G01N 33/566 (2006.01) G01N 33/68 (2006.01)
Patent
CA 2161265
A method of determining frost hardiness of a conifer seedling, which comprises detecting an amount of a cold protein in tissue of said conifer, the cold protein being a protein of approximately 19 kD that increase significantly in amount in the conifer during fall season (autumnal) months and that imparts frost hardiness to the seedling, and then assessing frost hardiness based on said detected amount. In the case of Pinus lambertiana, the cold protein includes the N-terminal sequence: Val-Ser-Gly- Thr-Ser-Ser-Thr-Glu-Glu- Val-Val-Gln-Asn-Glu-Ala-Arg-Arg-Leu-Trp-Asn [SEQ ID NO:1]; and in the case of Pinus monticola, the cold protein includes the N-terminal sequence: Val-Ser-Gly-Thr-Ser-Ser-Thr-Glu- Glu-Val-Val-Gln-Val-Glu-Ala-Arg-Arg-Leu-Trp-Asn-Ala-Thr- Thr-Lys-Asp [SEQ ID NO:3]. The invention also relates to conjugates and antibodies used in the method.
Anderson J. Wayne
As Represented By The Ministe R. Of Forestry Canada Her Majesty The Queen In Right Of Canada
Ekramoddoullah Abul K. M.
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