Test for glycosilated hemoglobins

G - Physics – 01 – N

Patent

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73/93

G01N 33/50 (2006.01)

Patent

CA 1167278

ABSTRACT OF THE DISCLOSURE The invention provides an improved procedure for the ion-exchange chromatographic separation of compounds, such as hemoglobins, by passing a separa- tor composition, which includes an ampholyte through the chromatography system prior to the introduction of the sample. When the assay is carried out using the separator composition as described in the pre- sent invention, the separation between HbA1 and HbA is improved such that the results obtained are inde- pendent of the temperature in a large range, i.e., at least from 15 to 35°C. The invention also provides a test kit. 096

400444

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