Test for periodontitis and susceptibility thereto

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/06 (2006.01) G01N 33/50 (2006.01) G01N 33/554 (2006.01) G01N 33/569 (2006.01) G01N 33/58 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2137680

Periodontitis and susceptibility thereto is tested by measuring the change in morphology of a sample of polymorphonuclear leukocytes (PMNs) from the patient at a predetermined time after incubation thereof with supernatant from a virulent strain of Porphyromonas gingivalis and comparing the result with that from a sam- ple of PMNs from a healthy patient. Since loss of polarisation corresponds to a loss of actin which is bound by phalloidin, the change can be measured by observing adecrease in fluorescence of fluorescently labelled phalloidin in the sample. In seri- ous cases there is a complete loss of fluorescence.

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