Test kit and method for competitive specific binding assay

Details Test kit and method for competitive specific binding assay Test kit and method for competitive specific binding assay

G - Physics

01

N

G01N 33/566 (2006.01) C12Q 1/28 (2006.01) G01N 33/543 (2006.01) G01N 33/88 (2006.01)

Patent

CA 2151690

One or more specific binding ligands can be detected with a competitive immunoassay which utilizes a water-soluble conjugate of ligand and reporter enzyme for each target ligand. Target ligand is allowed to compete for a first receptor with the conjugate. Uncomplexed conjugate is then contacted with an immobilized second receptor to form a reaction product which can be used to generate a detectable signal. This assay provides a direct correlation of the generated signal to the one or more target ligands.

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