Test kit for analysing factor viii-splitting protease

Details Test kit for analysing factor viii-splitting protease Test kit for analysing factor viii-splitting protease

G - Physics

01

N

G01N 33/86 (2006.01) C12Q 1/37 (2006.01)

Patent

CA 2362483

The invention relates to a test kit for analysing the von Willebrand factor- splitting protease and for carrying out differential diagnosis between patients with thrombotic thrombocytopenic purpura and patients with haemolytic- uraemic syndrome. Said test kit consists of a standard von Willebrand factor preparation which is free of von Willebrand factor-splitting activity, as a substrate for the von Willebrand factor-splitting activity in a sample or in the patient plasma, and a system for quantitatively determining the bonding of von Willebrand factor to collagen. The invention also relates to a method for detecting an acquired or congenital deficiency of von Willebrand factor- splitting protease.

Kit de test pour l'analyse de la protéase clivant le facteur Willebrand, qui permet d'établir le diagnostic différentiel entre des patients atteints de purpura thrombocytopénique thrombotique et des patients atteints du syndrome hémolytique et urémique. Ledit kit comporte une préparation standard de facteur Willebrand exempte d'activité de clivage du facteur Willebrand, en tant que substrat pour l'activité de clivage du facteur Willebrand dans un échantillon ou dans le plasma d'un patient, ainsi qu'un système de détermination quantitative de la liaison du facteur Willebrand à du collagène. La présente invention concerne également un procédé de détection d'un manque acquis ou congénital de la protéase clivant le facteur Willebrand.

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