Test method and device for total protein assay

G - Physics – 01 – N

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G01N 33/68 (2006.01) G01N 33/52 (2006.01)

Patent

CA 2001557

TEST METHOD AND DEVICE FOR TOTAL PROTEIN ASSAY Abstract of the Disclosure A test device and method of determining the presence and concentration of the total pro- tein content, including albumin and Bence Jones proteins, in a test sample. The test device in- cludes a test pad comprising a new and improved carrier matrix incorporating an indicator reagent composition capable of interacting with proteins to produce a visually or instrumentally detectable and/or measurable response. The new and improved carrier matrix of the test pad comprises a fibrous, bibulous substrate, such as filter paper, homogeneously impregnated with a polymerized urethane-based compound. The new and improved carrier matrix provides improved color resolution and increased sensitivity to proteins in dry phase test strip assays, thereby affording a more accurate and trustworthy protein assay of liquid test samples, such as urine. Furthermore, by utilizing a carrier matrix comprising a fibrous, bibulous substrate homogeneously impreg- nated with a polymerized urethane-based compound, a dry phase assay for low molecular weight pro- teins, such as Bence Jones proteins, can be per- formed on a liquid test sample. MS-1546

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