C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
150/3.2, 195/1.3
C12Q 1/68 (2006.01) C12N 1/20 (2006.01) C12Q 1/04 (2006.01) C12Q 1/12 (2006.01)
Patent
CA 1188239
ABSTRACT OF THE DISCLOSURE A strain of Neisseria gonorrhoeae ATCC 31953 is described which has characteristically poor growth on chocolate agar at a temperature range of about 30°C to about 37°C in a CO2 atmosphere suitable for growth of N. gonorrhoeae. This strain is resistant to nalidixic acid at the 5-10 mcg/ml level and resistant to streptomycin at the 1000 mcg/ml level or greater. N. gonorrhoeae ATCC 31953 is a test strain suitable for use in the method described for the laboratory diagnosis of gonorrhea. The method comprises the steps of (1) preparing a test strain of the microorgani ?sseria gonorrhoeae which is competent for transformation, (2) extracting N. gonorrhoeae DNA from a patient's s ?imen material or colonies suspected of being N. gonorrhoeae whether in pure or mixed culture by treatment with a base which lyses N. gonorrhoeae, (3) adusting the pH of the extract of step (2) to a pH that is not toxic to the test strain, (4) applying the pH adjusted extract to the test strain before or after putting it on or into a biological medium suitable for growth of N. gonorrhoeae, (5) maintaining the treated test strain at optimum N. gonorrhoeae growth conditions which are inhibitory for the test strain and (6) observing for detectable growth of the treated test strain. The observance of growth indicates positive detection of N. gonorrhoeae DNA. Also described is a test kit for performing the method described.
414224
Gowling Lafleur Henderson Llp
Temple University Of The Commonwealth System Of Higher Education
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