Test plate assembly defining discrete regions on a...

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G01N 33/50 (2006.01) B01D 61/18 (2006.01) C12M 1/34 (2006.01) C12Q 1/00 (2006.01) G01N 33/48 (2006.01)

Patent

CA 1286578

TEST PLATE ASSEMBLY DEFINING DISCRETE REGIONS ON A MICROPOROUS MEMBRANE WITH LOW BOUNDARY DISTORTION ABSTRACT OF THE DISCLOSURE A biochemical test plate assembly for use in multiple simultaneous contact tests arranged in a fixed array of discrete regions on a single microporous mem- brane sheet provides less distortion of the boundaries of these regions than do pre-existing assemblies. Like its predecessors, the assembly contains two apertured plates; an apertured gasket sheet and a microporous membrane, both placed between the apertured plates; and a third plate for placement beneath the apertured plates, having a recess which serves as a reservoir for liquids passing through the membrane. The improvement resides in the enclosure of the lower apertured plate by the upper apertured plate and the base plate, thereby seal- ing the lower apertured plate and the microporous mem- brane off entirely from the atmosphere. This eliminates evaporation of moisture from the edges of the membrane, and any lateral diffusion of biochemical species which might occur as a result due to capillary attraction.

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