Test set for the detection of antigen associated with...

G - Physics – 01 – N

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G01N 33/554 (2006.01) G01N 33/53 (2006.01)

Patent

CA 1111764

ABSTRACT OF THE DISCLOSURE This invention relates to a novel test set for detecting the presence of antigens associated with hepatitis in a test sample. The test comprises in combination, (a) an insoluble polymeric solid having antibody reactive with antigens associated with hepatitis bonded thereto for immobilizing hepatitis antigens from the sample, the surfaces on the solid being distorted to comprise a field of high and low points to provide a solid which, when placed in a flat- bottomed vial will be substantially in contact with any solution in the vial while minimizing surface-to-surface contact; (b) an alkaline phosphatase - antibody conjugate reagent for labelling hepatitis antigen immobilized on the solid; (c) an enzyme substrate capable of being chemically changed under the catalytic influence of the alkaline phosphatase; and (d) a flat-bottomed vial sized to reaceive the solid. The test is used in a sensitive direct immuno- assay system for detecting antigens associated with hepatitis involving the use of an enzyme-tugged antibody which reacts with an antigen to detect the presence of hepatitis. The test has an extended shelf life and the use thereof greatly simplifies the procedure of the assay.

351759

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