Test strip and method for determining ldl cholesterol...

G - Physics – 01 – N

Patent

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G01N 33/92 (2006.01) G01N 33/52 (2006.01)

Patent

CA 2499058

A dry phase test strip (20) and method are provided for determining the concentration of LDL in whole blood or plasma. The inventive test strip (20) includes one stack (92) or panel that measures concentration of total cholesterol and another stack (94) or panel that measures concentration of the sum total of HDL, VLDL and chylomicrons ("non-LDLs"). The difference between the values just noted is equal to the concentration of LDL cholesterol. Dry phase test strips (20) of the present invention function at room temperature and all test results are produced from pseudo-endpoint reflectance measurements such that the test method need not be timed. Also disclosed is the capability for an improved lipid panel that provides concentration in a blood sample of HDL, total cholesterol and LDL cholesterol without relying upon the Friedewald equation.

L'invention a trait à une bande d'essai en phase sèche (20) et à un procédé permettant de déterminer la concentration du cholestérol LDL dans un échantillon de sang total ou de plasma. La bande d'essai (20) selon l'invention comprend un empilement (92) ou une feuille, qui mesure la concentration du cholestérol total, et un autre empilement (94) ou feuille, qui mesure la concentration du cholestérol <= non LDL >=, soit la somme du cholestérol HDL, du cholestérol VLDL, et des chylomicrons. La différence entre les valeurs obtenues correspond à la concentration de cholestérol LDL. Les bandes d'essai en phase sèche (20) selon l'invention fonctionnent à température ambiante, et tous les résultats de l'essai sont produits à partir de mesures du facteur de réflexion effectuées lors d'un pseudo-point de virage, si bien qu'il n'est pas nécessaire de chronométrer le procédé d'essai. L'invention se rapporte également à la production d'une feuille à lipides améliorée, qui indique la concentration, dans un échantillon sanguin, du cholestérol HDL, du cholestérol total et du cholestérol LDL, et ce sans faire appel à la formule de Friedewald.

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