Testing individual immune response to an antibody with scid...

A - Human Necessities – 61 – K

Patent

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A61K 39/44 (2006.01) A01K 67/027 (2006.01)

Patent

CA 2144062

This patent application concerns a method for testing a patient's (P) individual immune response to an antibody "X" or binding fragment thereof which is specific for an epitope of a mediator of the patient's immune response against an antigen "Z" which method comprises the steps of: (i) isolating a sample of peripheral blood leucocytes (PBL) from the patient; (ii) en- grafting a mouse having severe combined immunodeficiency (SCID) with said sample in order to create a p-PBL-SCID test mouse having PBLs of the patient; (iii) treating said test mouse with a tolerogen designed for administration to the patient so as to reduce the patient's immune response to antibody "X"; (iv) after step (iii) immunising said test mouse with antibody "X"; (v) after (iii) and (iv) detecting any human antibody response in said test mouse; wherein the tolerogen comprises a water-soluble covalent conjugate of an antibody "Y" with one or more non-im- munogenic water soluble polymers and wherein said antibody "Y" although different from antibody "X" has structure in com- mon with it.

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