Texaphyrin metal complex mediated ester hydrolysis

C - Chemistry – Metallurgy – 07 – D

Patent

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Details

C07D 487/22 (2006.01) A61K 41/00 (2006.01) A61K 47/48 (2006.01) A61K 49/00 (2006.01) B01J 31/18 (2006.01) C07F 19/00 (2006.01) C07H 21/00 (2006.01) C07K 2/00 (2006.01) C07K 16/00 (2006.01) C12N 9/16 (2006.01) C12N 15/10 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2164419

A method of phosphate ester hydrolysis including incubating a solution of an aqueous phosphate ester with a texaphyrin metal complex. The metal is a metal cation having catalytic activity for ester bond hydrolysis in aqueous solution, in particular, a lanthanide metal cation, preferably Eu(III) or Dy(III). A preferred substrate is RNA and a preferred texaphyrin is a derivatized texaphyrin having binding specifically, in particular, a texaphyrin covalently coupled to a site-directed molecule, preferably an oligonucleotide.

Procédé d'hydrolyse des esters de phosphates, comprenant l'incubation d'une solution d'un ester de phosphate aqueux avec un complexe métallique de texaphyrine. Le métal utilisé se présente sous la forme d'un cation métallique ayant une activité catalytique provoquant l'hydrolyse des liaisons esters, en particulier un cation de métal appartenant aux lanthanides, de préférence Eu(III) ou Dy(III). Le substrat est de préférence de l'ARN et la texaphyrine est de préférence une texaphyrine dérivée ayant une spécificité de liaison, en particulier une texaphyrine présentant un couplage covalent avec une molécule dirigée vers un site, de préférence un oligonucléotide.

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