The generation of recombinant eukaryotic cells with...

C - Chemistry – Metallurgy – 12 – N

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195/1.13

C12N 15/85 (2006.01) C07K 14/755 (2006.01) C07K 14/81 (2006.01) C12N 5/10 (2006.01) C12N 15/65 (2006.01)

Patent

CA 1339207

-1- The generation of recombinant eukaryotic cells with high-level expression A method in which a considerable increase in the expression of one or more non-selectable genes can be brought about by cotransfection of at least two selectable genes and subsequent selection is described. In subsequent steps to increase the expression further, it is possible for selection for each gene marker to be carried out individually.

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