The human voltage gated sodium channel .beta.-1a subunit and...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/12 (2006.01) C07H 21/04 (2006.01) C07K 14/705 (2006.01) C07K 16/28 (2006.01) C12N 5/10 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2411069

DNAs encoding human voltage gated sodium channel .beta.1A subunit have been cloned and characterized. The recombinant protein is capable of forming biologically active protein. The cDNA's have been expressed in recombinant host cells that produce active recombinant protein. The recombinant protein is also purified from the recombinant host cells. In addition, the recombinant host cells are utilized to establish a method for identifying modulators of the receptor activity, and receptor modulators are identified.

Selon l'invention, des ADN codant pour des sous-unités .szlig.1A humaines du canal sodium électriquement potentialisé ont été clonées et caractérisées. La protéine de recombinaison est capable de former une protéine biologiquement active. Lesdits ADN ont été exprimés dans des cellules hôtes de recombinaison qui produisent une protéine active de recombinaison. Ladite protéine de recombinaison est également purifiée des cellules hôtes de recombinaison. En outre, les cellules hôtes de recombinaison servent à établir un procédé permettant d'identifier les modulateurs de l'activité du récepteur et les modulateurs du récepteur sont identifiés.

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