The mycolactone locus : an assembly line for producing novel...

C - Chemistry – Metallurgy – 12 – N

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Details

C12N 15/52 (2006.01) C12N 9/00 (2006.01) C12P 17/08 (2006.01) C12Q 1/68 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2546243

The present invention relates to Mycobacterium ulceran virulence plasmid, pMUM001 and particularly to a cluster of genes carried by this plasmid that encode polyketide synthases (PKSs) and polyketide-modifying enzymes necessary and sufficient for mycolactone biosynthesis. More particularly this invention is directed to novel purified or isolated polypeptides, the polynucleotides encoding such polypeptides, processes for production of such polypeptides, antibodies generated against these polypeptides, the use of such polynucleotides and polypeptides in diagnostic methods, kits, vaccines, therapy and for the production of mycolactone derivatives or novel polyketides by combinatorial synthesis.

La présente invention se rapporte à un plasmide de virulence de Mycobacterium ulcerans, pMUM001, et notamment à une famille de gènes portés par ce plasmid et codant les polycétide-synthases (PKS) et les enzymes modificatrices de polycétides nécessaires et suffisantes pour la synthèse de la mycolactone. La présente invention se rapporte plus spécialement à de nouveaux polypeptides purifiés ou isolés, aux polynucléotides codant pour ces polypeptides, à des procédés de production de polypeptides de ce type, à des anticorps dirigés contre ces polypeptides, à l'utilisation de polynucléotides et polypeptides de ce type dans les procédés diagnostiques, les trousses, les vaccins, les traitements et dans la production de dérivés de mycolactone ou de nouveaux polycétides par synthèse combinatoire.

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