The production and use of endophytes as novel inoculants for...

C - Chemistry – Metallurgy – 05 – F

Patent

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C05F 11/08 (2006.01) A01N 63/04 (2006.01) A01P 3/00 (2006.01) A01P 7/00 (2006.01) C12P 1/02 (2006.01)

Patent

CA 2646428

A process and method for the production of endophytes as plant inoculant products, specifically Clonostachys rosea strain 88- 710, for the promotion of plant vigor, health, growth and yield are disclosed. The endophyte, Clonostachys rosea strain 88- 710 produces a fungal conidial preparation by utilizing a discrete solid substrate fermentation system, namely Potato Dextrose Agar or Malt Extract Agar. Additionally, the endophyte, Clonostachys rosea strain 88-710, can act as an inoculant to stimulate and have an additive effect with rhizobium bacteria on the production of nitrogen fixing nodules on legumes and growth enhancment e.g. beans, soybeans, peas and alfalfa. As well, Clonostachys rosea strain 88-710, can combine with rooting hormones, e.g. indole-3 -butyric acid (IBA) to provide inoculant and rooting benefits to cuttings/transplants of plants.

La présente invention concerne un processus et un procédé destinés à la production d'endophytes, en particulier la souche Clonostachys rosea 88-710, utilisés comme produits inoculants pour le traitement des plantes en vue de favoriser la vigueur, la santé, la croissance et le rendement des végétaux. La souche endophyte Clonostachys rosea 88-710 produit une préparation conidiale fongique grâce à un système de fermentation sur substrat solide discret, à savoir de la gélose de pomme de terre et dextrose ou de la gélose à l'extrait de Malt. De plus, la souche endophyte Clonostachys rosea 88-710 peut, sous forme d'inoculant, agir avec les bactéries rhizobium pour stimuler et produire un effet additionnel sur le développement de nodules fixateurs d'azote des légumineuses et sur l'augmentation de la croissance des fèves, du soja, des pois et de l'alfalfa notamment. La souche Clonostachys rosea 88-710 peut également être combinée avec des hormones d'enracinement, l'acide indole-3-butyrique (IBA) par exemple, pour obtenir un produit inoculant ayant des effets bénéfiques d'enracinement sur les boutures et les plants repiqués.

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