C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
C07K 19/00 (2006.01) C12N 5/10 (2006.01) C12N 15/65 (2006.01) G01N 33/50 (2006.01)
Patent
CA 2687538
This invention relates to industrial production of proteins. More specifically, the invention relates to the res-DHFR surrogate marker, which corresponds to a fusion between DHFR and a protein conferring resistance to a toxic compound or conferring a metabolic advantage. The invention further relates to the use of res-DHFR for screening cells for high expression of a protein of interest. The invention is illustrated by the Puro-DHFR surrogate marker, which corresponds to a fusion between the puromycin N-acetyltransferase and dihydrofolate reductase (DHFR).
Cette invention concerne la production industrielle de protéines. Elle concerne plus particulièrement le marqueur de substitution res-DHFR, qui correspond à une fusion entre DHFR et une protéine conférant la résistance à un composé toxique ou conférant un avantage métabolique. L'invention concerne en outre l'utilisation de res-DHFR pour le criblage de cellules visant une haute expression d'une protéine d'intérêt. L'invention est illustrée par le marqueur de substitution Puro-DHFR, qui correspond à une fusion entre la puromycine N-acétyltransférase et la dihydrofolate réductase (DHFR).
Dupraz Philippe
Kobr Michel
Kirby Eades Gale Baker
Merck Serono S.a.
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