The recombinant production of proteins in yeast

Details The recombinant production of proteins in yeast The recombinant production of proteins in yeast

C - Chemistry, Metallurgy

12

N

C12N 15/81 (2006.01) C07K 14/815 (2006.01) C12N 9/34 (2006.01) C12N 15/62 (2006.01)

Patent

CA 2169567

Described is a method for the recombinant preparation of proteins in Hansenula yeast, the method being characterized in that Hansenula is transformed using an expression cassette which includes encoded in it the following structural elements: L - A - P - GEN in which L is a leader sequence, A is an adaptor generating an alpha-helix structure, P is a processing signal and GEN is a structural gene for the protein required.

L'invention concerne un procédé de préparation de protéines par recombinaison dans la levure d'Hansenula, qui se caractérise en ce que l'Hansenula est transformée à l'aide d'une cassette d'expression qui contient sous forme codée les éléments structuraux suivants: L - A - P - GEN, L étant une séquence leader, A étant un adaptateur générant une structure en hélice alpha, P étant un signal de maturation et GEN étant un gène structural de la protéine voulue.

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