C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/82 (2006.01) A01H 5/00 (2006.01) C12N 15/29 (2006.01)
Patent
CA 2372859
The current invention provides regulatory regions from the rice actin 2 gene. In particular, the current invention provides the rice actin 2 promoter and actin 2 intron. Compositions comprising these sequences are described, as well as transformation constructs derived therefrom. Further provided are methods for the expression of transgenes in plants comprising the use of these sequences. The methods of the invention include the direct creation of transgenic plants with the rice actin 2 intron and/or promoter directly by genetic transformation, as well as by plant breeding methods. The actin 2 sequences of the invention represent a valuable new tool for the creation of transgenic plants, preferably having one or more added beneficial characteristics.
La présente invention concerne des régions régulatrices issues du gène actine 2 du riz, et plus particulièrement le promoteur actine 2 du riz et l'intron actine 2. On décrit des compositions comprenant ces séquences ainsi que des constructions de transformation dérivées de ces dernières. On décrit également des procédés d'expression de transgènes dans des plantes dans lesquels on utilise ces séquences. Les procédés selon la présente invention comprennent la création directe de plantes transgéniques avec l'intron actine 2 du riz et/ou le promoteur directement par transformation génétique, ainsi qu'au moyen de procédés d'amélioration et de sélection des plantes. Les séquences actine 2 selon l'invention constituent un nouvel outil intéressant pour créer des plantes transgéniques, ayant de préférence au moins une caractéristique supplémentaire avantageuse.
Mcelroy David
Wu Ray
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Cornell Research Foundation Inc.
Dekalb Genetics Corporation
Monsanto Technology Llc
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