The taga gene and methods for detecting predisposition to...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/31 (2006.01) C07K 14/205 (2006.01) C12N 1/21 (2006.01) C12Q 1/68 (2006.01) G01N 33/569 (2006.01) A61K 38/00 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2201353

The present invention provides an isolated nucleic acid encoding an approximately 120-128 kilodalton antigen of Helicobacter pylori, or an antigenic fragment thereof, wherein the antigen is associated with peptic ulceration. The present invention also provides methods of detecting the presence of a Helicobacter pylori strain possessing the 120-128 kilodalton antigen in a subject, comprising the steps of contacting an antibody- containing sample from the subject with a detectable amount of the tagA antigen or antigenic polypeptide of the present invention and detecting the binding of the antigen or fragment and the antibody. The detection of a strain expressing the TagA antigen is an indication of predisposition to peptic ulceration and gastric carcinoma. A mutant H. pylori not expressing a functional TagA antigen is also provided.

La présente invention concerne un acide nucléique isolé codant pour un antigène d'environ 120-128 kilodaltons d'Helicobacter pylori ou un fragment antigénique de celui-ci, l'antigène étant associé aux ulcères gastroduodénaux. La présente invention concerne également des procédés de détection de la présence d'une souche d'Helicobacter pylori possédant l'antigène de 120-128 kilodaltons chez un sujet, consistant à mettre en contact un échantillon contenant un anticorps, provenant d'un sujet, avec une quantité détectable de l'antigène tagA ou du polypeptide antigénique de la présente invention et à détecter la fixation de l'antigène ou du fragment sur l'anticorps. La présence d'une souche exprimant l'antigène TagA est une indication d'une prédisposition aux ulcères gastroduodénaux et aux carcinomes gastriques. L'invention concerne également un mutant d'H. pylori n'exprimant pas un antigène TagA fonctionnel.

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