The thermo-inducible expression of heterologous genes in...

Details The thermo-inducible expression of heterologous genes in... The thermo-inducible expression of heterologous genes in...

C - Chemistry, Metallurgy

12

N

195/1.2, 195/1.2

C12N 15/75 (2006.01) C12N 1/21 (2006.01) C12N 15/73 (2006.01) C12P 21/00 (2006.01)

Patent

CA 2022135

- 21 - ABSTRACT OF THE DISCLOSURE The regulation of the expression of a heterologous gene which codes for a protein of interest in B.subtilis is achieved by the introduction into the microorganism of a plasmid vector containing the gene placed under the control of a strong promoter whose activity is modulated by means of a variation in the temperature by the heat-sensitive repressor of the lambda phage of E.coli. The method enables various heterologous proteins to be synthesised by the culture of strains of B.subtilis with transformations induced by variations in temperature.

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