The use of homologous amds genes as selectable markers

Details The use of homologous amds genes as selectable markers The use of homologous amds genes as selectable markers

C - Chemistry, Metallurgy

12

N

C12N 15/55 (2006.01) C12N 1/15 (2006.01) C12N 1/19 (2006.01) C12N 9/80 (2006.01) C12N 15/64 (2006.01) C12N 15/65 (2006.01) C12N 15/80 (2006.01) C12N 15/90 (2006.01) C12P 1/00 (2006.01) C12P 21/02 (2006.01) C12Q 1/34 (2006.01)

Patent

CA 2201730

The present invention discloses novel amdS genes from fungi previously not known to contain and amdS gene, such as Aspergillus niger and Penicillium chrysogenum. The novel amdS genes can be used as homologous selectable marker genes in the transformation of these fungi. Alternatively, the cloned amdS genes can be used to inactivate the endogenous copy of the gene in order to reduce the background in transformation experiments.

L'invention se rapporte à un nouveau gène amdS de champignons dont on ne savait pas précédemment qu'ils contenaient un gène amdS, à savoir par exemple: Aspergillus niger et Penicillium chrysogenum. Le nouveau gène amdS peut être utilisé comme marqueur sélectable homologue dans la transformation desdits champignons. Dans un autre mode de réalisation, les gènes amdS clonés peuvent être utilisés pour inactiver la copie endogène du gène en vue d'affaiblir l'arrière-plan des expériences de transformation.

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