The use of modular oligonucleotides as probes or primers in...

Details The use of modular oligonucleotides as probes or primers in... The use of modular oligonucleotides as probes or primers in...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2266749

The invention provides a method of improving the binding of a series of consecutive nucleotide bases to a complementary target nucleic acid molecule in a sample, wherein said method comprises at least the step or steps of binding a complementary modular oligonucleotide of at least two parts (modules) including said nucleotide bases to adjacent stretches of said target nucleic acid molecule in said sample, especially methods of detection/isolation and method in which the modular oligonucleotide is a primer, modular oligonucleotides themselves and their use in methods of the invention.

L'invention concerne un procédé visant à améliorer la liaison d'une série de bases nucléotides successives à une molécule d'acide nucléique cible complémentaire dans un échantillon. Ledit procédé comprend au moins une ou plusieurs étapes consistant à lier un oligonucléotide modulaire complémentaire, qui est composé d'au moins deux parties (modules) comprenant lesdites bases nucléotides, à des extensions contiguës de ladite molécule d'acide nucléique cible dudit échantillon. L'invention concerne en particulier des procédés de détection/isolation et des procédés dans lesquels l'oligonucléotide modulaire est une amorce. L'invention concerne en outre des oligonucléotides modulaires et leur utilisation dans des procédés décrits par l'invention.

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