C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C07H 21/00 (2006.01) C07K 5/06 (2006.01) C07K 7/06 (2006.01) C07K 7/08 (2006.01) C07K 14/00 (2006.01) C08G 69/00 (2006.01) C12Q 1/70 (2006.01) A61K 38/00 (2006.01)
Patent
CA 2109805
-114- THE USE OF NUCLEIC ACID ANALOGUES IN DIAGNOSTICS AND ANALYTICAL PROCEDURES ABSTRACT Methods of capture, recognition, detection, identification or quantitation of nucleic acids and diagnostics uses generally are described in which are used (a) a peptide nucleic acid (PNA) comprising a polyamide backbone bearing a plurality of ligands at respective spaced locations along said backbone, said ligands being each independently naturally occurring nucleobases, non-naturally occurring nucleobases or nucleobase-binding groups, each said ligand being bound directly or indirectly to a nitrogen atom in said backbone, and said ligand bearing nitrogen atoms mainly being separated from one another in said backbone by from 4 to 8 intervening atoms; (b) a nucleic acid analogue capable of hybridising to a nucleic acid of complementary sequence to form a hybrid which is more stable against denaturation by heat than a hybrid between the conventional deoxyribonucleotide corresponding to said analogue and said nucleic acid; or (c) a nucleic acid analogue capable of hybridising to a double stranded nucleic acid in which one strand has a sequence complementary to said analogue, so as to displace the other strand from said one strand. Preferred compounds have the formula:- -115- Image wherein: each L is independently selected from the group consisting of hydrogen, phenyl, naturally occurring nucleobases, and non-naturally occurring nucleobases; each R7' is independently selected from the group consisting of hydrogen and the side chains of naturally occurring alpha amino acids; n is an integer from 1 to 60, each k and m is, independently, zero or one; and each 1 is independently from zero to 5; Rh is OH, NH2 or -NHLysNH2; and Ri is H or COCH3.
Berg Rolf H.
Buchardt Ole
Egholm Michael
Nielsen Peter E.
Berg Rolf H.
Buchardt Dorte
Buchardt Ole
Egholm Michael
Nielsen Peter E.
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