Therapeutic angiogenesis by bone marrow-derived cell...

C - Chemistry – Metallurgy – 12 – N

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Details

C12N 5/0786 (2010.01) C12N 5/071 (2010.01) A61K 35/28 (2006.01) A61K 48/00 (2006.01) A61P 9/00 (2006.01) C12N 5/10 (2006.01)

Patent

CA 2429502

The invention provides methods of forming new blood vessels in diseased or damaged tissue in a subject, methods of increasing blood flow to diseased or damaged tissue in a subject, and methods of increasing angiogenesis in diseased tissue in a subject, which methods comprise: a) isolating autologous bone marrow-mononuclear cells from the subject; b) transplanting locally into the diseased or damaged tissue an effective amount of the autologous bone- marrow mononuclear cells, thereby forming new blood vessels in the diseased or damaged tissue. The new blood vessels may be capillaries or collateral vessels in ischemic tissue or any site of angiogenesis. Also provided are methods of treating tissue in disease or injury by local transplantation with an effective amount of the autologous bone marrow-mononuclear cells so as to induce vascularization in such diseased tissue.

Cette invention concerne des méthodes pour former de nouveaux vaisseaux sanguins dans un tissu malade ou endommagé d'un sujet, et des méthodes pour augmenter le débit sanguin dans le tissu malade ou endommagé. Elle concerne également des méthodes pour augmenter l'angiogenèse dans un tissu malade d'un sujet, qui consistent à: a) isoler chez le sujet des cellules mononucléaires de moelle osseuse autologue; b) greffer localement dans le tissu malade ou endommagé une quantité effective des cellules mononucléaires de moelle osseuse autologue afin d'y former de nouveaux vaisseaux sanguins. Les nouveaux vaisseaux sanguins peuvent être des capillaires ou des vaisseaux collatéraux dans le tissu ischémique ou dans un site quelconque de l'angiogenèse. L'invention concerne en outre des méthodes de traitement d'un tissu malade ou d'une blessure par greffe locale avec une quantité effective des cellules mononucléaires de moelle osseuse autologue de façon à induire la vascularisation dans ledit tissu.

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