Therapeutic blood product means and methods of preparing same

Details Therapeutic blood product means and methods of preparing same Therapeutic blood product means and methods of preparing same

C - Chemistry, Metallurgy

12

N

195/78, 195/39.1

C12N 9/64 (2006.01) A61K 35/16 (2006.01) A61K 38/00 (2006.01)

Patent

CA 1288711

ABSTRACT OF THE DISCLOSURE A method of producing highly purified factor IX from crude factor 12 comprising binding an A-7 monoclonal antibody to an Affigel*-10 column; partially purifying the crude factor IX to produce a partially purified factor IX; applying that factor IX to the A-7-Affigel*-10 column with an application buffer; washing the column with 1-3M NaCl in 0.05M Tris*-HCl, 20 mM MgCl2 and 1 mM Benzamidine HCl; eluting purified factor IX from the washed column with 20 mM EDTA; and separating purified factor IX from the EDTA. An improved factor IX product produced thereby. * trade mark

526744

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