Therapeutic fragments of von willebrand factor

C - Chemistry – Metallurgy – 12 – N

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C12N 15/12 (2006.01) A61K 38/37 (2006.01) A61K 39/395 (2006.01) C07K 14/755 (2006.01) C07K 16/36 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2107100

2107100 9217192 PCTABS00016 A polypeptide patterned on a fragment of wild type mature von Willebrand factor (vWF) subunit having one ore more binding sites of predetermined affinity for one or more of the ligands selected from the group consisting of collagen, glycosaminoglycans, proteoglycans, platelet glycoprotein Ib.alpha., platelet glycoprotein IIb/IIIa, or coagulation factor VIII, said polypeptide having a modified amino acid sequence relative to that of said fragment and an increased binding affinity, relative to said predetermined affinity, for one or more of said ligands, including also such a polypeptide prepared by mutagenesis of a DNA sequence and patterned on wild type mature vWF subunit, and also a polypeptide in purified form patterned upon a parent polypeptide which comprises the wild type amino acid sequence of mature von Willebrand factor subunit, or a fragment thereof, and including also purified DNA sequences encoding such polypeptides, expression plasmids and viral expression vectors containing the DNA sequences, and therapeutic compositions comprising such polypeptides effective in the treatment of thrombosis, and methods for the use thereof, and also preparation of such polypeptides by mutagenesis of an encoding DNA sequence or covalent modification of wild type vWF.

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