Therapeutic fragments of von willebrand factor

C - Chemistry – Metallurgy – 12 – N

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C12N 15/12 (2006.01) A61K 38/37 (2006.01) A61K 39/395 (2006.01) C07K 14/755 (2006.01) C07K 16/36 (2006.01) C12N 1/19 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12P 21/02 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2094259

2094259 9206999 PCTABS00012 A polypeptide patterned upon a parent polypeptide and comprising the amino acid sequence of that fragment of mature von Willebrand factor subunit which begins approximately at residue 441 (arginine) and ends at approximately residue 733 (valine), or any subset thereof, in which one or more of the cysteine residues normally present in the parent polypeptide, or subset thereof, have been deleted and/or replaced by one or more other amino acids, said polypeptide having therefore less tendency than the parent polypeptide, or subset thereof, to form intra or interchain disulfide bonds in aqueous media at a physiological pH, and including also a DNA sequence encoding an aforementioned polypeptide; and also a biologically functional expression plasmid or viral expression vector containing DNA encoding for an aforementioned polypeptide and capable of being replicated in a host cell; a therapeutic composition comprising one or more of the involved polypeptides; and also a method for inhibiting thrombosis in a patient which comprises administering to such patient an effective amount of the therapeutic composition.

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