A - Human Necessities – 01 – N
Patent
A - Human Necessities
01
N
A01N 61/00 (2006.01) A01N 1/02 (2006.01) A01N 37/18 (2006.01) A01N 43/04 (2006.01) A61K 35/14 (2006.01) A61K 47/48 (2006.01) C12N 5/00 (2006.01) C12N 15/63 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2397362
A dehydrated composition is provided that includes freeze-dried platelets. The platelets are loaded with trehalose which preserves biological properties during freeze-drying and rehydration. The trehalose loading is conducted at a temperature of from greater than about 25 ~C to less than about 40 ~C, most preferably at 37 ~C, with the loading solution having trehalose in an amount from about 10 mM to about 50 mM. These freeze-dried platelets are substantially shelf-stable and are rehydratable so as to have a normal response to an agonist, for example, thrombin, with virtually all of the platelets participating in clot formation within about three minutes at 37 ~C.
L'invention concerne une composition déshydratée contenant des plaquettes lyophilisées. Les plaquettes sont chargées de tréhalose conservant les propriétés biologiques durant la lyophilisation et la réhydratation. La charge de tréhalose est effectuée à une température supérieure à environ 25 ·C et inférieure à environ 40 ·C, de préférence à 37 ·C, la solution de charge contenant une quantité de tréhalose située entre environ 10 mM et 50 mM. Ces plaquettes lyophilisées ont essentiellement une longue durée de conservation et peuvent être réhydratées de manière à présenter une réponse normale par rapport à un agoniste, par exemple la thrombine, quasiment toutes les plaquettes participant à la formation de caillots en l'espace de 3 minutes à 37 ·C.
Crowe John H.
Oliver Ann E.
Tablin Fern
Walker Naomi J.
Wolkers Willem F.
Smart & Biggar
The Regents Of The University Of California
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