C - Chemistry – Metallurgy – 12 – M
Patent
C - Chemistry, Metallurgy
12
M
C12M 1/40 (2006.01) C12M 1/36 (2006.01) C12P 19/34 (2006.01)
Patent
CA 2267708
A thermal cycler for performing amplification of nucleic acids. In order to provide fully automatic motion and positioning of a lid (14) suitable for heating a plurality of a reaction vessels (13) and for shielding them from environment light, the thermal cycler comprises (a) a thermal block (71) having a plurality of chambers (72) each of which is apt to receive the lower part of a reaction vessel (13); (b) a movable lid (14) having heating means (15) positioned within it, said lid (14) having a surface apt to cover said thermal block (71) and reaction vessels (13) positioned in said chambers (72) thereof, said lid (14) being positionable at a first position, at which it covers said thermal block (71) and reaction vessels positioned in said chambers thereof, and at a second position, at which said lid (14) does not cover said thermal block (71) and reaction vessels positioned in said chambers thereof, and (c) transport means for moving said lid (14) from said first position to said second position and viceversa, the path followed by the lid (14) as it is moved from said second position to said first position comprises: a first path segment which lies above and at a predetermined distance from said thermal block (71) and along which said lid (14) is moved towards a stopper (16) until it abuts against said stopper, and a second path segment along which said lid (14) is moved away from said stopper (16) and downwards until it is vertically aligned with said thermal block (71) and exerts a predetermined pressure on reaction vessels (13) positioned in said chambers (72) of said thermal block.
F. Hoffmann-La Roche Ag
Gowling Lafleur Henderson Llp
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