Thermal cycling reaction block and continuous real-time...

C - Chemistry – Metallurgy – 12 – M

Patent

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C12M 1/38 (2006.01)

Patent

CA 2728203

Provided is a real-time monitoring apparatus, including a thermal cycling reaction block having heating block which is formed of a hollow part and divided by an insulating layer, and a capillary tube through which a sample is flowed in and/or out and which is wound on the heating block so that different temperatures are transferred and thus reaction cycle is repeatedly performed a light source; a band pass filter; a condensing lens; a beam splitter a reflecting mirror which is rotatably connected with a motor so as to transfer the excitation light reflected from the beam splitter to the capillary tube and reflect the fluorescence generated from the sample in the capillary tube; and a fluorescence detecting part.

Linvention concerne un dispositif de surveillance en temps réel comprenant un bloc réactionnel de cycle thermique comportant un bloc chauffant qui est composé dune partie creuse et divisé par une couche isolante, et un tube capillaire à travers lequel un échantillon entre et/ou sort et qui est enroulé sur le bloc chauffant de façon à transférer différentes températures et donc à réaliser de manière répétée le cycle réactionnel ; une source de lumière ; un filtre passe-bande ; un condenseur ; un séparateur de faisceau ; un miroir réfléchissant qui est relié de manière pivotante à un moteur de façon à transférer la lumière dexcitation réfléchie depuis le séparateur de faisceau vers le tube capillaire et à réfléchir la fluorescence générée de léchantillon dans le tube capillaire ; et une partie détectant la fluorescence.

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