C - Chemistry – Metallurgy – 12 – M
Patent
C - Chemistry, Metallurgy
12
M
C12M 1/00 (2006.01) C12M 1/02 (2006.01) C12M 3/00 (2006.01)
Patent
CA 2445362
The invention concerns a cell and tissue culture device comprising at least a culture well (18-i), first (2)and second (25) reservoirs each housing at least a flexible pouch (6, 7; 27, 29) one of which at least can receive a culture fluid, linking means (20, 21) coupled to the well and the pouches for circulating the culture fluid from one reservoir to the other via the well, pressurising means (60-92) for applying to the pouches of the first (2) and second (25) reservoirs respectively first and/or second external pressure sequence(s) defined by a control module (50) and designed to control the culture fluid circulation in the well, temperature control means (49, 51-58) monitored by the control module and designed to maintain a first selected temperature inside the well and to set at a second selected temperature the culture fluid emerging from the first and second reservoirs for supplying the well.
Un dispositif de culture de cellules et tissus comprend au moins un puits de culture (18-i), de premier (2) et second (25) réservoirs logeant chacun au moins une poche souple (6,7; 27, 29) dont l'une au moins peut recevoir un fluide de culture, des moyens de liaison (20, 21) couplés au puits et aux poches pour la circulation du fluide de culture d'un réservoir à l'autre via le puits, des moyens de pressurisation (60-92) pour appliquer aux poches des premier (2) et second (25) réservoirs respectivement une première et/ou une seconde séquences de pressions externes définies par un module de commande (50) et destinées à gérer la circulation du fluide de culture dans le puits, et des moyens de régulation thermique (49, 51-58) pilotés par le module de commande et agencés pour maintenir une première température choisie à l'intérieur du puits et placer sous une seconde température choisie le fluide de culture qui débouche des premier et second réservoirs pour alimenter le puits.
Sarem Farzin
Sarem Damerdji Leila-Ouassila
Smart & Biggar
Societe Nouvelle Cell Tissue Progress
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